Hi there I have this code:
from functools import partial
import multiprocessing
def kmer_count(sequence, alphabet, k):
"""Returns a dictionary with kmers and it counts."""
seq = sequence.upper()
seq_len = len(seq)
kmers = [seq[i:i+k] for i in range(0, seq_len - k + 1)]
filterd_kmers = [kmer for kmer in kmers if
all(base in set(alphabet) for base in kmer)]
return Counter(filterd_kmers)
pool = multiprocessing.Pool(4)
args = [''.join(alphabet.iupac_dna), 4] # in the real code it came from argparse
f = partial(kmer_count,seq)
pool.starmap(f, *args)
Result:
---------------------------------------------------------------------------
RemoteTraceback Traceback (most recent call last)
RemoteTraceback:
"""
Traceback (most recent call last):
File "/opt/anaconda3/lib/python3.7/multiprocessing/pool.py", line 121, in worker
result = (True, func(*args, **kwds))
File "/opt/anaconda3/lib/python3.7/multiprocessing/pool.py", line 47, in starmapstar
return list(itertools.starmap(args[0], args[1]))
TypeError: kmer_count() missing 1 required positional argument: 'k'
"""
The above exception was the direct cause of the following exception:
TypeError Traceback (most recent call last)
<ipython-input-79-0f5ba8381a51> in <module>
1 f = partial(kmer_count,seq)
----> 2 pool.starmap(f, *args)
/opt/anaconda3/lib/python3.7/multiprocessing/pool.py in starmap(self, func, iterable, chunksize)
274 func and (a, b) becomes func(a, b).
275 '''
--> 276 return self._map_async(func, iterable, starmapstar, chunksize).get()
277
278 def starmap_async(self, func, iterable, chunksize=None, callback=None,
/opt/anaconda3/lib/python3.7/multiprocessing/pool.py in get(self, timeout)
655 return self._value
656 else:
--> 657 raise self._value
658
659 def _set(self, i, obj):
TypeError: kmer_count() missing 1 required positional argument: 'k'
If I run like this:
f(*args)
I got this:
Counter({'GTTC': 9780,
'TTCG': 11291,
'TCGC': 14803,
'CGCC': 14428,
'GCCA': 19254,
'CCAG': 11512,
'CAGA': 11641,
'AGAG': 10387,
'GAGC': 13164,
'AGCG': 16025,
'GCGG': 13067,
'CGGT': 12329,
'GGTT': 14217,
'GTTT': 18733,
'TTTT': 25488,
'TTTG': 22006,
'TTGA': 20004,
'TGAC': 11351,
'GACT': 7262,
'ACTA': 5663,
'CTAG': 4190,
'TAGC': 8543,
'AGCT': 12898,
'GCTT': 19114,
'CTTG': 16557,
'TGAA': 17703,
'GAAC': 9334,
'AACA': 14050,
'ACAC': 8812,
'CACA': 11050,
'ACAT': 9705,
'CATC': 17357,
'ATCC': 11157,
'TCCC': 5754,
'CCCG': 5926,
'CCGT': 8601,
'CGTC': 7401,
'GTCC': 3811,
'TCCT': 5890,
'CCTG': 7985,
'CTGC': 15324,
'TGCG': 14856,
'TTTA': 15607,
'TTAG': 8366,
'TAGG': 5393,
'AGGC': 9580,
'GGCA': 13939,
'GCAC': 11686,
'CACC': 14496,
'ACCA': 16950,
'CCAC': 13028,
'CACT': 13194,
'ACTT': 12585,
'TTGC': 18877,
'TGCA': 13770,
'GCAT': 13307,
'CATT': 14102,
'ATTA': 9776,
'TTAA': 12585,
'TAAG': 9048,
'AAGC': 18834,
'AGCC': 12734,
'CCAT': 13799,
'CATA': 8188,
'ATAA': 12278,
'GCGT': 12546,
'CGTT': 12599,
'GTTG': 15136,
'TTGG': 18903,
'TGGC': 19391,
'GGCC': 7685,
'GCCC': 7404,
'CCCA': 9752,
'CCAA': 18755,
'CAAC': 14852,
'AACT': 11886,
'ACTC': 10028,
'CTCA': 13518,
'TCAC': 15385,
'ACCG': 12027,
'CCGC': 13105,
'CGCA': 14853,
'ATAG': 7066,
'AGGG': 5273,
'GGGG': 4927,
'GGGT': 7972,
'GAAA': 16511,
'AAAC': 18215,
'ACTG': 12639,
'TGCT': 16663,
'CTTT': 18228,
'TTTC': 17497,
'TCGG': 10116,
'TGAT': 20593,
'GATG': 17301,
'ATGA': 14218,
'GATC': 14479,
'ATCG': 16604,
'CGCG': 13405,
'CGTA': 8086,
'GTAG': 6829,
'TAGA': 6656,
'AGAT': 11833,
'ATCA': 20344,
'TCAG': 13868,
'ATCT': 11892,
'TCTT': 14706,
'CTTC': 13897,
'TTCC': 9522,
'TCCG': 7099,
'CCGG': 5935,
'CGGG': 5786,
'GGGA': 5615,
'GGAT': 11054,
'GATA': 12085,
'ATAT': 9205,
'TATC': 11941,
'TCGT': 8802,
'GTAT': 8661,
'TATT': 12620,
'ATTT': 18149,
'TGAG': 13150,
'GAGA': 8658,
'GAGG': 5644,
'GCCT': 9673,
'CCTT': 11377,
'CTTA': 9100,
'TTAC': 11418,
'TACG': 8250,
'ACGC': 12774,
'CACG': 10171,
'ACGA': 8951,
'CGAT': 16617,
'ATTG': 17372,
'GGCG': 14257,
'TTGT': 13287,
'TGTA': 7808,
'ACGG': 8529,
'CGGA': 7109,
'ATGT': 9975,
'TGTC': 8286,
'GTCG': 9088,
'TCGA': 11986,
'GATT': 16232,
'GACC': 7608,
'CATG': 11552,
'ATGG': 13703,
'TGGG': 9728,
'CGAC': 9020,
'GACG': 7394,
'ACGT': 8085,
'GTAA': 11360,
'TAAT': 9895,
'AATA': 12342,
'GGCT': 12713,
'AAAT': 17834,
'AATC': 16220,
'TTCA': 18526,
'TCAA': 20304,
'CAAA': 21752,
'AATG': 14200,
'TGGT': 16952,
'GTTA': 10179,
'GCGA': 14624,
'AGGT': 9338,
'GGTA': 10326,
'CGGC': 12423,
'TAAC': 9956,
'AACG': 12524,
'GCGC': 18306,
'CTGG': 11344,
'TGGA': 10805,
'GCTG': 17455,
'CTGA': 13532,
'CGAG': 8649,
'GAGT': 10014,
'AGTA': 8668,
'GTAC': 8038,
'TACC': 10217,
'ACCT': 9204,
'ATAC': 8527,
'TACA': 7453,
'ACAA': 12591,
'AAAA': 24650,
'AAAG': 17848,
'AAGG': 11240,
'AGTT': 12431,
'CAAT': 17456,
'GTCT': 6235,
'AGGA': 5937,
'GGAG': 5294,
'AGAA': 12516,
'AAGA': 13980,
'CGTG': 9948,
'GTGC': 11762,
'TCAT': 14388,
'AGTC': 7451,
'GTCA': 11557,
'CAGT': 13149,
'GGTC': 7553,
'GTGA': 15043,
'TAAA': 15634,
'GGGC': 7200,
'GGTG': 14495,
'TTCT': 13230,
'TCTA': 6621,
'CTAC': 6510,
'TACT': 8573,
'CTAT': 6856,
'TATG': 7993,
'ATTC': 11395,
'GAAG': 13449,
'AAGT': 12578,
'GCTA': 8346,
'CTAA': 8310,
'TAGT': 5859,
'GACA': 8060,
'TTAT': 12293,
'TCCA': 11138,
'CAGC': 17389,
'GCTC': 13661,
'CTCG': 8724,
'CCCC': 5036,
'TGCC': 13824,
'GCCG': 12340,
'GGAA': 9364,
'CTGT': 9651,
'TGTT': 14580,
'ATGC': 13150,
'AGTG': 13050,
'GTGT': 9052,
'TCTG': 11772,
'AATT': 13738,
'CGAA': 11026,
'GTGG': 12927,
'TCTC': 8866,
'ACAG': 9505,
'CCTC': 5686,
'CTCC': 5391,
'CCTA': 5236,
'CGCT': 16302,
'AACC': 13897,
'ACCC': 8037,
'GCAA': 18697,
'CAAG': 16287,
'AGCA': 16273,
'GCAG': 15145,
'CCGA': 9751,
'CAGG': 7851,
'TATA': 4461,
'GGAC': 3754,
'CTCT': 10608,
'TGTG': 11291,
'AGAC': 6199,
'CCCT': 5517,
'GAAT': 11315}
What I am doing wrong with the multiprocessing step. I want to speed up my analysis, because I have a lot of genomes in a directory to process. I just need to figure out how can I implement the pool, because probably all other functions a similar.
Thanks for your time and attention
