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Simply I have a function which looks like this: $$\lambda =\lambda_0 \cdot \exp(-T_0/T)\;,$$, where $\lambda_0$ and $T_0$ are unknown constants. By appplying to both sides ln() I get : $$\ln(\lambda)=\ln(\lambda_0)-T_0(1/T)$$ It's of form $y=b+a/x$. The question is how can I apply linear regression formulae? Maybe smart substitution will be enough ?

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Yes, if you take $\frac{1}{T}$ as predictor and $\ln(\lambda)$ as response, you just have a very usual linear regression problem.

Anyway, you should remember to check linear regression assumptions with the transformed variables.

Pere
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    Note that $T_0$ is unknown so you can't use it to make a predictor. (You may want to read whuber's comment under the question as well) – Glen_b Feb 21 '17 at 02:41