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In what cases should I use a comma after “please”?

Do I have to use comma after "please" in the beginning of the sentence? E.g. "Please, specify the reason." or "Please specify the reason."

algot
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  • I'd say separate please with commas because it is an interjection if it is at the end of a sentence. – Luke_0 Aug 15 '12 at 14:27
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    But Wikipedia's article about interjection says different :) http://screencast.com/t/4nGop9Y3VP – algot Aug 15 '12 at 14:29

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