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Is it: The programs will take place at 9 a.m., 11 a.m. and noon

Or: The programs will take place at 9 a.m., 11 a.m., and noon

Chris
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    It depends entirely on what style guide you follow—if you use a serial comma or not. – Jason Bassford Jul 02 '18 at 23:06
  • Jason's right. It depends on what style guide you follow. It's not a question of grammar but a question of style. The comma in question is an Oxford comma, sometimes called a serial comma. Whether or not you include it is personal preference unless you are to follow a particular style guide, like that of your employer or university, which style guides always have a position on this and explicitly say whether you are to include it or not. – Billy Jul 02 '18 at 23:49

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My preference is for:

The programs will take place at 9 a.m., 11 a.m., and noon.

I prefer the so-called "Oxford comma," as a rule of thumb, because in some instances the lack thereof can result in confusion.