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I am wondering if I mix 1 tbs of honey, ACV and olive oil with 1 crushed garlic with a squirt of lemon juice, then microwave it for 15 seconds (enough make the mixture bubble but not overflow) can that cause botulism if I immediately drink the mixture afterwards?

Violet
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No, of course not. Botulism is the result of bacterial fermentation over a long period of time. If the ingredients are safe, mixing them will also be safe.

Sneftel
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    I would suggest adding the corollary that if the ingredients were not safe in the first place, then microwaving them will not "kill the spores". – Federico Poloni Apr 06 '22 at 09:23
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    @FedericoPoloni That's not what the OP was asking about. None of the OP's ingredients are "suspect". (FWIW, though, boiling for long enough will inactivate botulinum toxin, which is all that matters for eating it.) – Sneftel Apr 06 '22 at 10:31
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    @FedericoPoloni The spores are not dangerous to >6 months olds, or we would all be dead by now. Bacteria and viruses, on the other hand, will suffer a lot from 15 seconds of boiling (if I understand the OP right). It's basically a flash pasteurization that just falls a bit short of industry standards. – Peter - Reinstate Monica Apr 06 '22 at 13:21
  • Thanks @Sneftel and Peter for the corrections; this seems useful information. – Federico Poloni Apr 06 '22 at 13:24
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    Might want to clarify "a long period of time". Botulism can poison a mixture in as little as 72 hours. Much more than the OP is asking about, but folks may refer to your answer out of context. – FuzzyChef Apr 06 '22 at 16:23
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    @Sneftel Honey and garlic absolutely can contain C botulinum. It just is usually not harmful to adults. – BlackThorn Apr 06 '22 at 20:21
  • @FedericoPoloni I've heard/read that you should avoid using fresh garlic in sous vide recipes because of the risk of botulin poisoning. But the time in question doesn't seem to be of concern. – JimmyJames Apr 06 '22 at 21:10
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    @Peter-ReinstateMonica The toxin is destroyed by heat but the spores are heat resistant and the spores will turn into more C. botulinum over time in oil. That's why it's important to make the distinction that it's safe to consume immediately but storage is more difficult (microwaving does not make it safe to store). – user3067860 Apr 07 '22 at 17:36